Mitosis and the spindle checkpoint

May, K.M., Paldi, F., Hardwick, K.G. (2017).  Fission Yeast Apc15 Stabilises MCC-Cdc20-APC/C Complexes, Ensuring Efficient Cdc20 Ubiquitination and Checkpoint Arrest.  Current Biology

Yuan, I., Leontiou, I., Amin, P., May, K., Chafraidh, S.S., Zlamalova, E. and Hardwick, K.G. (2016).  Generation of a spindle checkpoint arrest from synthetic signalling assemblies.  Current Biology, 27

Zich J., May K., Paraskevopoulos K., Sen O., Syred H.M., van der Sar S.J., Patel H., Moresco J.L., Sarkeshik A., Yates III J.R., Rappsilber J., and Hardwick K.G. (2016).  Mps1Mph1 kinase phosphorylates Mad3 to inhibit Cdc20Slp1-APC/C and maintain spindle checkpoint arrests.  PLoS Genetics,12(2), e1005834.

Folco H.D., Campbell C.S., May K.M., Espinoza C.A., Oegema K., Hardwick K.G., Grewal S.I., Desai A. (2015).  The CENP-A N-tail confers epigenetic stability to centromeres via the CENP-T branch of the CCAN in fission yeast.  Current Biology, 25, 348-356.

Paraskevopoulos K, Kriegenburg F, Tatham M.H., Rösner H.I., Medina B., Larsen I.B., Brandstrup R., Hardwick K.G., Hay R.T., Kragelund B.B., Hartmann-Petersen R., Gordon C.  (2014).  Dss1 is a 26S proteasome ubiquitin receptor.  Molecular Cell, 56, 453-461.

Vanoosthuyse, V., Legros, P., van der Sar, S.J., Yvert, G., Toda, K., Le Bihan, T., Watanabe, Y., Hardwick, K., and Bernard, P. (2014).  CPF-associated phosphatase activity opposes condensin-mediated chromosome condensation.  PLoS Genetics, 10, e1004415.

Kim, H.S., Mukhopadhyay, R., Rothbart, S.B., Silva, A.C., Vanoosthuyse, V., Radovani, E., Kislinger, T., Roguev, A., Ryan, C.J., Xu, J., Jahari, H., Hardwick, K.G., Greenblatt, J.F., Krogan, N.J., Fillingham, J.S., Strahl, B.D., Bouhassira, E.E., Edelmann, W., and Keogh, M.C. (2014).  Identification of a BET family bromodomain/casein kinase II/TAF-containing complex as a regulator of mitotic condensing function.  Cell Reports, 6, 892-905.

Patel, H., Zich, J., Serrels, B., Rickman, C., Hardwick, K.G., Frame, M.C., and Brunton, V.G. (2013).  Kindlin-1 regulates mitotic spindle formation by interacting with integrins and Plk-1.  Nature Communications, 4, 2056.

Shepperd, L.A., Meadows, J.C., Sochaj, A.M., Lancaster, T.C., Zou, J., Buttrick, G.J., Rappsilber, J., Hardwick, K.G. and Millar, J.B.A. (2012).  Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint.  Current Biology, 22, 891-9.

Zich, J., Sochaj, A.M., Syred, H.M., Milne, L., Cook, A.G., Ohkura, H., Rappsilber, J. and Hardwick, K.G. (2012).  Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex.  Current Biology, 22, 296-301.

Buttrick, G.J., Meadows, J.C., Lancaster, T.C., Vanoosthuyse, V., Shepperd, L.A., Hoe, K.L., Kim, D.U., Park, H.O., Hardwick, K.G., and Millar, J.B. (2011). Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B. Molecular Biology of the Cell, 22, 4486-4502.

Meadows, J.C., Shepperd, L.A., Vanoosthuyse, V., Lancaster, T.C., Sochaj, A., Buttrick, G.J., Hardwick, K.G. and Millar, J.B.A. (2011).  Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors.  Developmental Cell 20, 739-750.

Hardwick, K.G and Shah, J.V. (2010).  Spindle checkpoint silencing: ensuring rapid and concerted anaphase onset.  F1000 Reports 2:55.

Vanoosthuyse, V. and Hardwick, K.G. (2009).  Overcoming inhibition in the spindle checkpoint.  Genes and Development 23, 2799-2805.

Kim, H.S., Vanoosthuyse, V., Fillingham, J. Roguev, A., Watt, S., Kislinger, T., Treyer, A., Carpenter, L.R., Bennett, C.S., Emili, A., Greenblatt, J.F., Hardwick, K.G., Krogan, N., Bahler, J., and Keogh, M.C. (2009).  An acetylated form of H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe.  Nature Structural and Molecular Biology, 16, 1286-1293.

Vanoosthuyse, V., Meadows, J.C., van der Sar, S.J.A., Millar, J.B.A. and Hardwick, K.G. (2009)  Bub3p facilitates spindle checkpoint silencing in fission yeast. Molecular Biology of the Cell, 20, 5096-5105.

Zich, J. and Hardwick, K.G. (2009).  Getting down to the phosphorylated “nuts and bolts” of spindle checkpoint signalling.  Trends in Biochemistry, 35, 18-27.

Vanoosthuyse, V. and Hardwick, K.G. (2009). A novel Protein Phosphatase 1-dependent spindle checkpoint silencing mechanism.  Current Biology, 19, 1176-81.

Fernius, J. and Hardwick, K.G. (2009). The spindle checkpoint: assays for the analysis of spindle checkpoint arrest and recovery.  Methods in Molecular Biology, 545, 243-258.

Sczaniecka, M., Feoktistova, A., May, K.M., Chen, J.S., Blyth, J., Gould, K.L., and Hardwick, K.G. (2008).  The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-APC/C.  Journal of Biological Chemistry 283, 23039-47.

Sczaniecka, M.M. and Hardwick, K.G. (2008).  The spindle checkpoint: how do cells delay anaphase onset?  SEB Experimental Biology Series, 59, 243-256.

Rischitor, P., May, K.M. and Hardwick, K.G. (2007). Bub1 is a fission yeast kinetochore scaffold protein, and is sufficient to recruit other spindle checkpoint proteins to ectopic sites on chromosomes.  PLoS ONE 2(12): e1342.

Fernius, J. and Hardwick, K.G. (2007). Bub1 Kinase Targets Sgo1 to Ensure Accurate Chromosome Biorientation and Segregation in Budding Yeast Mitosis.  PLoS Genetics 3(11): e213.

King, E.M.J., Rachidi, N., Morrice, N., Hardwick, K.G., and Stark, M.J.R. (2007).  Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.  Genes and Development, 21, 1163-8.

King, E.M.J., van der Sar, S.J.A., and Hardwick, K.G. (2007).  Mad3 KEN boxes mediate both Cdc20 and Mad3 turnover, and are critical for the spindle checkpoint.  PLoS ONE 2(4): e342.

Anderson, V.E., Prudden, J., Prochnik, S., Giddings, T., and Hardwick, K.G. (2007).  Novel sfi1 alleles uncover additional functions for Sfi1p in bi-polar spindle assembly and function.  Molecular Biology of the Cell, 18, 2047-56.

Vanoosthuyse, V., Prykhozhij, S., and Hardwick, K.G. (2007). Shugoshin2 regulates localisation of the Chromosomal Passenger proteins in fission yeast mitosis.  Molecular Biology of the Cell 18, 1657-79.

May, K.M., and Hardwick. K.G. (2006).  The Spindle Checkpoint.  Journal of Cell Science, 119, 4139-42.

Vaur, S., Cubizolles, F., Plane, G., Genier, S., Rabitsch, P.K., Gregan, J., Nasmyth, K., Vanoosthuyse, V., Hardwick, K.G. and Javerzat, J.P. (2005).  Control of shugoshin function during fission yeast meiosis.  Current Biology 15, 2263-70.

Vanoosthuyse, V. and Hardwick, K.G. (2005).  Bub1 and the multi-layered inhibition of Cdc20-APC/C in mitosis.  Trends in Cell Biology, 15, 231-233.

Hardwick, K.G. (2005).  Checkpoint signalling: Mad2 conformers and signal propagation.  Current Biology, 15, R122-3.

Kadura S, He X, Vanoosthuyse V, Hardwick KG, Sazer S. (2005).  A78V Mutation in the Mad3-like Domain of S. pombe Bub1p Perturbs Nuclear Accumulation and Kinetochore Targeting of Bub1p, Bub3p, and Mad3p and Spindle Assembly Checkpoint Function.  Molecular Biology of the Cell, 16, 385-395.

Vanoosthuyse V, Valsdottir R, Javerzat JP, Hardwick KG. (2004).  Kinetochore targeting of fission yeast Mad and Bub proteins is essential for spindle checkpoint function but not for all chromosome segregation roles of Bub1p.  Molecular and Cellular Biology, 24, 9786-801.

Campbell, L. and Hardwick, K.G. (2003).  Analysis of Bub3 spindle checkpoint function in Xenopus egg extracts.  Journal of Cell Science, 116, 617-628.

Vanoosthuyse, V. and Hardwick, K.G. (2003).  The complexity of Bub1 regulation:  phosphorylation, phosphorylation, phosphorylation?  Cell Cycle 2, 118-119.

Musacchio, A. and Hardwick, K.G. (2002).  The spindle checkpoint:  structural insights into dynamic signalling.  Nature Reviews in Molecular and Cellular Biology, 3, 731-741.

Warren, C.D., Brady, D.M., Johnston, R.C., Hanna, J.S., Hardwick, K.G., and Spencer, F.A.  (2002). Distinct chromosome segregation roles for spindle checkpoint proteins.  Molecular Biology of the Cell, 13, 3029-3041.

Millband, D.N. and Hardwick, K.G. (2002). Fission yeast Mad3p is required for Mad2p to inhibit the APC, and localises to kinetochores in a Bub1p, Bub3p and Mph1p dependent manner.  Molecular and Cellular Biology, 22, 2728-2742.

Millband, D.N., Campbell, L., and Hardwick, K.G. (2002). The awesome power of multiple model systems: interpreting the complex nature of spindle checkpoint signalling. Trends in Cell Biology 12, 205-209.

Brady, D.M., and Hardwick, K.G. (2000). Complex formation between Mad1p and Bub1p is crucial for spindle checkpoint function.  Current Biology 10, 675-678.

Rudner, A.D., Hardwick, K.G., and Murray, A.W. (2000). Cdc28 activates exit from mitosis in budding yeast.  Journal of Cell Biology 149, 1361-1376.

Hardwick, K.G., Johnston, R.C., Smith, D.L. and Murray, A.W.. (2000) MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p and Mad2p.  Journal of Cell Biology 148, 871-882.

Chen, R-H., Brady, D.M., Smith, D. Murray, A.W. and Hardwick, K.G.. (1999).  The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins. Molecular Biology of the Cell 10, 2607-2618.

Hardwick, K.G., Li, R., Mistrot, C., Chen, R-H., Dann, P., Rudner, A., and Murray, A.W. (1999).  Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiaeGenetics 152, 509-518.

Bernard, P., Hardwick, K.G., and Javerzat, J.-P. (1998).  Fission yeast Bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis.  Journal of Cell Biology 143, 1775-87.

Hwang L.H., Lau, L.F., Smith, D.L., Mistrot, C.A., Hardwick, K.G., Hwang, E.S., Amon, A., and Murray, A.W. (1998). Budding yeast Cdc20: a target of the spindle checkpoint.  Science 279, 1041-44.

Hardwick, K.G. (1998).  The spindle checkpoint.  Trends in Genetics 14, 1-4.

Hardwick, K.G., Weiss, E., Luca, F., Winey, M., and Murray, A.W. (1996).  Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption.  Science 273, 953-956.             

Hardwick, K.G. and Murray, A.W. (1995).  Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast. J. Cell Biol. 131, 709-720. 


Protein sorting (PhD)

SED6 is identical to ERG6, and encodes a putative methyltransferase required for ergosterol synthesis. (1994) K G Hardwick, H R Pelham. Yeast 10(2):265-9

SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex. (1992) K G Hardwick, H R Pelham. J. Cell Biol. 119(3):513-21

Genes that allow yeast cells to grow in the absence of the HDEL receptor. (1992) K G Hardwick, J C Boothroyd, A D Rudner, H R Pelham. EMBO J. 11(11):4187-95

ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway. (1990) J C Semenza, K G Hardwick, N Dean, H R Pelham. Cell 61(7):1349-57

ERS1 a seven transmembrane domain protein from Saccharomyces cerevisiae. (1990) K G Hardwick, H R Pelham. Nucleic Acids Res. 18(8):2177

ERD1, a yeast gene required for the retention of luminal endoplasmic reticulum proteins, affects glycoprotein processing in the Golgi apparatus. (1990) K G Hardwick, M J Lewis, J Semenza, N Dean, H R Pelham. EMBO J. 9(3):623-30

Sorting of soluble ER proteins in yeast. (1988) H R Pelham, K G Hardwick, M J Lewis. EMBO J. 7(6):1757-62